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SF501 serum-free medium for LMH cells

A serum-free medium especially developed for efficient large-scale culture of LMH cells

SF501 medium is an innovative serum-free medium developed independently by Shanghai BioEngine Sci-Tech Co., Ltd., which is serum-free, protein-free, and animal-derived component-free. It is the first medium for serum-free total suspension culture of LMH cells in China. It supports efficient acclimation of adherent LMH cells and high-density growth of suspension cells and provides a high-quality environment for efficient expression of avian adenovirus vaccines. SF501 is your ideal choice for large-scale industrial suspension culture of LMH cells: the suspension culture system provides you with a significant increase in production efficiency, while the serum-free culture system provides you with maximum process stability. Currently, it has been applied on a large scale in a number of avian adenovirus vaccine production projects.

SF501 serum-free medium for LMH cells

Features

Serum-free

Animal-derived component-free

Protein-free

Support the suspension adaption of LMH cells

Support the high cell-density culture of LMH cells

Support high-efficiency amplification of avian adenovirus

Advantages

The first medium in China for industrial application of avian adenovirus production based on serum-free suspension culture of LMH cells;

Full traceability by EU-certified ISO13485:2016 Quality Management System;

Excellent inter-batch consistency (CPK*>1.33);

Optional powder media for use in large-scale manufacturing with easy preparation procedures;

Powder media capable of a single batch size of 100,000 L.*CPK: Process Capability Index. CPK>1.33 indicates good process control and small inter-batch difference of products.

Order Information
Basal Medium
ApplicationProduct NameCat. No.SizeSizeProduct Instruction (pdf)Inquiries /Sample application
Support high productivity of avian adenovirus vaccinea
SF501 LMH Serum-free Medium
EXP0105703
200 L Powder Inquiries /Sample application
EXP0105701
100 L Powder
EXP0105702
10 L Powder
EXP0100901
1 L Liquid
*DrivingM® is a registered trademark of Shanghai BioEngine Sci-Tech Co., Ltd.
Performance
Vaccines

LMH adherent cells could be directly acclimated in SF501 without serum for rapid adaptation to the suspension culture system.

LMH cells were acclimated from adherent state to suspension culture, and they were individually dispersed and translucent in morphology with a uniform cell size.

Successfully acclimated LMH suspension cells were inoculated at 1×106 cells/ml in SF501, and LMH cell density reached 5-7×106 cells/ml in 72 h, with high viability for long-term stable passages.

LMH cells were inoculated at 1×106 cells/ml in SF501 and reached a maximum density of 2×107 cells/ml with a plateau period of up to 4 days.

Using SF501 suspension culture of LMH for serum-free production of avian adenovirus (serotype 4) achieved the highest titer of 109.5-10.1 TCID50/ml, comparable to that of adherent serous culture (108.7-9.3 TCID50/ml). Suspension culture allows for a significant increase in production efficiency.

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We have a professional technical team to provide high quality one-stop cell culture service from process development
and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

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BioEngine

3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

(86)21-68582660-2792

globalsales@bio-engine.com.cn

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